Mechanism of porphobilinogen synthase. Possible role of essential thiol groups.
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چکیده
منابع مشابه
Mechanism of porphobilinogen synthase. Possible role of essential thiol groups.
The inactivation of beef liver porphobilinogen synthase by the alkylating agents iodoacetate and iodoacetamide has been investigated. The synthase can be inactivated by these reagents only if the enzyme has been activated initially by sulfhydryl reducing agents. Inactivation by both iodoacetate and iodoacetamide was the result of modification of cysteinyl residues. A plot of the incorporation o...
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The N-terminal sequence of porphobilinogen-synthase (EC 4.2.1.24) from bovine liver up to position 44 is given. After tryptic hydrolysis two N-terminal peptides with identical amino acid composition were isolated in the ratio 1:1. One peptide was blocked whereas the other one started with methionine and showed the same primary structure which had been estimated by automatic degradation of the e...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1977
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)38333-3